Screening of Potential Actinomycetes Isolates against Selective Pathogenic Microorganism

Objective: To isolate, evaluate and characterize the potential bioactive compound producing actinomycetes from soil sample of different location of Gwalior. Method: A total of 11 strains of actinomycetes were isolated from soil collected from different location of Gwalior. The isolates were characterized by using morphological and biochemical methods. 11 isolates were identified and subjected to submerged fermentation methods to produce crude extracts. The fermented biomass was extracted by organic solvent extraction method and tested against bacterial strains by agar well diffusion method. Results: All the isolates of this study were gram positive and their colony morphology was studied microscopically. Morphologically Isolates had branched mycelium. All 11 isolates were biochemically characterized. Majority of isolates (90.90%) were able to hydrolysis of starch; 18.18% isolates were produced proteolytic caseinase and utilize casein as carbon source efficiently; all isolates were negative for indole production; 81.81% isolates were positive for citrate utilization test, all isolates were negative for gas and hydrogen sulphide production while 18.18% isolates were found positive for glucose fermentation and 27.2% for sucrose fermentation. In this study it has been found that 27.27% isolates were reported with broad spectrum antibacterial activity. In this study it has been found that GWL 3 and GWL 6 demonstrate highest bactericidal activity against Salmonella typhi with 15mm ZOI. This study also concludes that maximum number of isolates exhibit antibacterial activity against Salmonella typhi. KeywordsActinomycetes, Bioactive, Biochemical, Submerged.